CD47 anticorps

N° du produit ABIN120599

L’anticorps Souris Monoclonal anti-CD47 a été validé pour FACS, WB et IHC (fro). Il convient pour détecter CD47 dans des échantillons de Humain.

Aperçu rapide pour CD47 anticorps (ABIN120599)

Antigène: CD47

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-122

Détail du produit anti-CD47 anticorps

Specificité: The antibody reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (Thrombospondin Receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

Purification: CHT-Chromatography and Precipitation Methods

Immunogène: COS-7 (African green monkey) cells

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry. Western blotting (non-reducing conditions). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody at 2 - 8 °C. Do not freeze.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CD47

Antigène: CD47

Autre désignation: CD47

Sujet: CD47 is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein a, SIRPa), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.Synonyms: Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA3

ID gène: 961

UniProt: Q08722