CD59 anticorps

N° du produit ABIN120611

L’anticorps Souris Monoclonal anti-CD59 a été validé pour WB, FACS, IP et IHC (p). Il convient pour détecter CD59 dans des échantillons de Humain.

Aperçu rapide pour CD59 anticorps (ABIN120611)

Antigène: CD59

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD59 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-43-5

Détail du produit anti-CD59 anticorps

Specificité: The antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues. The antibody does not compete with most other CD59 antibodies.

Purification: Protein-A affinity chromatography

Pureté: > 95 % pure (by SDS-PAGE)

Immunogène: Thymocytes and T lymphocytes

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections) Recommended dilution: 5 m g/mL. Western Blotting: 1-2 μg/mL (non-reducing conditions). Flow Cytometry: 1 μg/mL. Positive control: blood. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CD59

Antigène: CD59

Autre désignation: CD59

Sujet: CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin

ID gène: 966

UniProt: P13987

Pathways: Système du Complément