CD63 anticorps

N° du produit ABIN120614

L’anticorps Souris Monoclonal anti-CD63 a été validé pour FACS, WB, IF, IP et IHC (p). Il convient pour détecter CD63 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour CD63 anticorps (ABIN120614)

Antigène: CD63

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-259

Détail du produit anti-CD63 anticorps

Specificité: The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: HPB-ALL T cell line

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry (detection of activated platelets, neutrophils and basophils). Immunoprecipitation. Western blotting under non-reducing conditions. Immunocytochemistry. Immunohistochemistry (paraffin sections): Recommended dilution:10 m g/mL, Positive tissue: spleen
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Date de péremption: 12 months

Références pour anticorps anti-CD63 (ABIN120614)

Schmidt, Gelhaus, Nebendahl, Lettau, Lucius, Leippe, Kabelitz, Janssen: "Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells." dans: Cell communication and signaling : CCS, Vol. 9, Issue 1, pp. 4, (2011) (PubMed).

Détails sur CD63

Antigène: CD63

Autre désignation: CD63

Sujet: CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.Synonyms: Granulophysin, ME491, MLA1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, TSPAN30, Tetraspanin-30

ID gène: 967

UniProt: P08962