LIME anticorps (C-Term)

N° du produit ABIN120742

Détail du produit anti-LIME anticorps

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Épitope: AA 281-296, C-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIME est non-conjugé

Application: Western Blotting (WB)

Specificité: The antibody reacts with the cytoplasmic domain of LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Purification: Protein A affinity chromatography

Pureté: > 95 % (by SDS-PAGE)

Immunogène: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin

Clone: LIME10

Isotype: IgG2a

Information d'application

Indications d'application: Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur LIME

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Autre désignation: LIME1 (LIME1 Produits)

Synonymes: anticorps LIME1, anticorps DKFZp459A158, anticorps LIME, anticorps LP8067, anticorps dJ583P15.4, anticorps 2810038K19Rik, anticorps RGD1562795, anticorps Lck interacting transmembrane adaptor 1, anticorps LIME1, anticorps Lime1

Sujet: LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

ID gène: 54923

UniProt: Q9H400