LIME anticorps (Intracellular)

N° du produit ABIN120743

L’anticorps Souris Monoclonal anti-LIME a été validé pour IP. Il convient pour détecter LIME dans des échantillons de Humain.

Aperçu rapide pour LIME anticorps (Intracellular) (ABIN120743)

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIME est non-conjugé

Application: Immunoprecipitation (IP)

Clone: LIME-06

Détail du produit anti-LIME anticorps

Épitope: AA 141-295, Intracellular

Specificité: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Purification: Protein A affinity chromatography

Pureté: > 95 % by SDS-PAGE

Immunogène: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur LIME

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Autre désignation: LIME1

Sujet: LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

ID gène: 54923

UniProt: Q9H400