CSK anticorps (AA 330-450)

N° du produit ABIN120744

Détail du produit anti-CSK anticorps

Antigène: CSK (C-Src tyrosine Kinase (CSK))

Épitope: AA 330-450

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CSK est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificité: The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.

Purification: Protein A affinity chromatography

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450

Clone: CSK-04

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation. Western Blotting (1:250-1:300. Weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions). Immunochemistry (Positive control: HeLa human cervix carcinoma cell line).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CSK

Antigène: CSK (C-Src tyrosine Kinase (CSK))

Autre désignation: CSK (CSK Produits)

Synonymes: anticorps AW212630, anticorps C-terminal Src kinase, anticorps c-src tyrosine kinase, anticorps CSK, non-receptor tyrosine kinase, anticorps CSK, anticorps Csk

Sujet: C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.Synonyms: C-SRC kinase, Protein-tyrosine kinase CYL, Tyrosine-protein kinase CSK

ID gène: 1445

UniProt: P41240

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events