SOCS3 anticorps

N° du produit ABIN120754

Cet anticorps Souris Monoclonal détecte spécifiquement SOCS3 dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour SOCS3 anticorps (ABIN120754)

Antigène: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOCS3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 01-11-14

Détail du produit anti-SOCS3 anticorps

Specificité: This antibody SO1 reacts with SOCS3, an intracellular Cytokine Signaling Inhibitor.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Affinity Chromatography on Protein A

Immunogène: Full-length SOCS3 protein

Isotype: IgG2b

Information d'application

Indications d'application: ELISA. Western blot: Use HeLa human cervix carcinoma cell line as positive control. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including protease inhibitor cocktail). Incubate 60 min on ice, centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 15 mM Sodium Azide as preservative, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur SOCS3

Antigène: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Autre désignation: SOCS3 / CIS3

Sujet: SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.Synonyms: CIS-3, Cytokine-inducible SH2 protein 3, SOCS-3, SSI-3, SSI3, STAT-induced STAT inhibitor 3, Suppressor of cytokine signaling 3

ID gène: 9021

UniProt: O14543

Pathways: Signalistation JAK/STAT, Response to Growth Hormone Stimulus, Hepatitis C