PUMA anticorps (N-Term)

N° du produit ABIN121685

L’anticorps Lapin Polyclonal anti-PUMA a été validé pour WB et IHC (p). Il convient pour détecter PUMA dans des échantillons de Humain et Souris.

Aperçu rapide pour PUMA anticorps (N-Term) (ABIN121685)

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUMA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PUMA anticorps

Épitope: N-Term

Specificité: This antibody recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogène: Synthetic peptide corresponding to the N-terminus of human PUMA alpha. AA Sequence: ARARQEGSSPEPVEG

Isotype: IgG

Information d'application

Indications d'application: Western blot: 2 μg/mL - 4 μg/mL, this antibody detects a band of approximately 23 kDa in K562 cell lysates. Immunohistochemistry on paraffin sections: 10 μg/mL, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur PUMA

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Autre désignation: PUMA

Sujet: PUMA is a proapoptotic member of the BCL-2 family. PUMA is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

UniProt: Q9BXH1

Pathways: Signalisation p53, Positive Regulation of Endopeptidase Activity