CD14 anticorps

N° du produit ABIN126002

L’anticorps Souris Monoclonal anti-CD14 a été validé pour FACS, WB, IP, Func et EIA. Il convient pour détecter CD14 dans des échantillons de Humain et Primate.

Aperçu rapide pour CD14 anticorps (ABIN126002)

Antigène: CD14

Reactivité: Humain, Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: MEM-18

Détail du produit anti-CD14 anticorps

Purification: Protein A Chromatography

Immunogène: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry. Immunoprecipitation. Western blotting (non-denaturing conditions). ELISA: antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with clone B-A8 (ABIN120555). Functional application: completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Azide Free Phosphate Buffered Saline (PBS), pH ~7.4, 0.2 μm filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Agent conservateur: Azide free

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades