c-FOS anticorps (N-Term)

N° du produit ABIN126801

Détail du produit anti-c-FOS anticorps

Antigène: c-FOS (c-Fos) (c-Fos)

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp c-FOS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: This antibody specifically interacts with the N-terminus of c-Fos. The antibody is suitable for Western blot and ELISA applications. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.

Attributs du produit: Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Synthetic peptide conjugated to KLH.

Clone: 8B5

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch

Date de péremption: 12 months

Détails sur c-FOS

Antigène: c-FOS (c-Fos) (c-Fos)

Autre désignation: C-Fos (c-Fos Produits)

Synonymes: anticorps AP-1, anticorps C-FOS, anticorps p55, anticorps c-fos, anticorps D12Rfj1, anticorps cFos, anticorps cFOS, anticorps fos, anticorps Fos proto-oncogene, AP-1 transcription factor subunit, anticorps FBJ osteosarcoma oncogene, anticorps proto-oncogene c-Fos-like, anticorps FOS, anticorps Fos, anticorps LOC101078177

Sujet: The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

ID gène: 2353

UniProt: P01100

Pathways: S100 Proteins