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VASP anticorps (pSer157)

VASP Reactivité: Humain, Souris WB, IF, IP, FACS, EIA Hôte: Souris Monoclonal 5C6 unconjugated
N° du produit ABIN126908
  • Antigène Voir toutes VASP Anticorps
    VASP (Vasodilator-Stimulated phosphoprotein (VASP))
    Épitope
    • 18
    • 17
    • 15
    • 10
    • 9
    • 8
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer157
    Reactivité
    • 137
    • 64
    • 58
    • 12
    • 11
    • 9
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Souris
    Hôte
    • 116
    • 20
    • 2
    Souris
    Clonalité
    • 111
    • 27
    Monoclonal
    Conjugué
    • 80
    • 10
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp VASP est non-conjugé
    Application
    • 105
    • 51
    • 50
    • 27
    • 22
    • 17
    • 14
    • 14
    • 11
    • 11
    • 5
    • 4
    • 4
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    Specificité
    AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
    Attributs du produit
    Synonyms: Vasodilator-stimulated phosphoprotein
    Purification
    Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
    Immunogène
    Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 157 RRVpSNAG
    Clone
    5C6
    Isotype
    IgG1
    Top Product
    Discover our top product VASP Anticorps primaire
  • Indications d'application
    Western blot ( 0.5 μg/mL for HRPO/ECL detection). Recommended: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA (0.05 μg/mL). Immunoprecipitation (1 - 10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1 - 10 μg/mL, may tolerate 0.5 % formaldehyde fixation). Flow cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    PBS with 0.09 % Sodium Azide, PEG and Sucrose
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!
    Date de péremption
    12 months
  • Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).

  • Antigène
    VASP (Vasodilator-Stimulated phosphoprotein (VASP))
    Autre désignation
    VASP (VASP Produits)
    Synonymes
    anticorps vasp, anticorps MGC80889, anticorps wu:fb23b04, anticorps wu:fk84g05, anticorps zgc:110347, anticorps si:dkey-113g17.1, anticorps si:ch211-202c21.1, anticorps DDBDRAFT_0188463, anticorps DDBDRAFT_0229340, anticorps DDB_0188463, anticorps DDB_0229340, anticorps VASP, anticorps vasodilator stimulated phosphoprotein S homeolog, anticorps vasodilator stimulated phosphoprotein b, anticorps protein enabled, anticorps vasodilator-stimulated phosphoprotein, anticorps vasodilator stimulated phosphoprotein a, anticorps vasodilator stimulated phosphoprotein, anticorps vasp.S, anticorps vaspb, anticorps LOC5573842, anticorps CpipJ_CPIJ004706, anticorps CpipJ_CPIJ004707, anticorps vasP, anticorps vasp, anticorps VASP, anticorps vaspa, anticorps Vasp
    Sujet
    VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Molecular Weight: 46/50 kDaSynonyms: Vasodilator-stimulated phosphoprotein
    ID gène
    7408
    UniProt
    P50552
    Pathways
    TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
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