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NSF anticorps

L’anticorps Lapin Polyclonal anti-NSF a été validé pour WB, IP et IHC (p). Il convient pour détecter NSF dans des échantillons de Rat et Souris.
N° du produit ABIN127184

Aperçu rapide pour NSF anticorps (ABIN127184)

Antigène

Voir toutes NSF Anticorps
NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivité

  • 50
  • 30
  • 30
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Rat, Souris

Hôte

  • 60
  • 6
  • 1
Lapin

Clonalité

  • 63
  • 4
Polyclonal

Conjugué

  • 34
  • 5
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp NSF est non-conjugé

Application

  • 55
  • 26
  • 13
  • 13
  • 11
  • 10
  • 10
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Specificité

    The antibody recognizes 85 kDa NSF.

    Purification

    Protein A chromatography

    Immunogène

    Synthetic peptide corresponding to amino acids around 731 of rat NSF
  • Indications d'application

    Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-15 mg/mL. Immunohistochemistry: 10-20 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.
  • Antigène

    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

    Autre désignation

    NSF

    Sujet

    Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.Synonyms: N-ethylmaleimide-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

    ID gène

    60355

    NCBI Accession

    NP_068516

    UniProt

    Q9QUL6
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