CDK1 anticorps

N° du produit ABIN129504

N° du produit (Fournisseur): 200-301-160

Cet anticorps anti-CDK1 est un anticorps Souris Monoclonal détectant CDK1 dans WB, ELISA et IP. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour CDK1 anticorps (ABIN129504)

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: POH-1

Détail du produit anti-CDK1 anticorps

N° du produit (Fournisseur): 200-301-160

Fournisseur: Rockland

Fonction: Cdc2 p34 Antibody

Réactivité croisée (Details): This antibody is not cross reactive with other cyclin dependent kinases.

Attributs du produit: Synonyms: mouse anti-Cdc2 antibody, mouse anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, CDC28A, CDKN1, P34CDC2

Purification: This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.

Immunogen Type: Recombinant Protein

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Application Note: Anti-p34 cdc2 has been tested by western blot and is suitable for immunohistochemistry, immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control.   Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CDK1 (ABIN129504)

Ye, Fincher, Tang, McNeal, Gygax, Wexler, Ryan, James, Osmani: "Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2." dans: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 33384-93, (1998) (PubMed).

Palmer, Gavin, Nebreda: "A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1." dans: The EMBO journal, Vol. 17, Issue 17, pp. 5037-47, (1998) (PubMed).

Détails sur CDK1

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: CDK1

Sujet: Background: p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphorylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.

ID gène: 983

NCBI Accession: NP_001307847

UniProt: P06493

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA