PMS2 anticorps

N° du produit ABIN129513

N° du produit (Fournisseur): 200-301-864

Cet anticorps anti-PMS2 est un anticorps Souris Monoclonal détectant PMS2 dans WB et ELISA. Adapté pour Humain.

Aperçu rapide pour PMS2 anticorps (ABIN129513)

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PMS2 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 1204

Détail du produit anti-PMS2 anticorps

N° du produit (Fournisseur): 200-301-864

Fournisseur: Rockland

Fonction: PMS2 Antibody

Réactivité croisée (Details): The epitope was putatively mapped to amino acids 58-81 of human PMS2.

Attributs du produit: Synonyms: mouse anti-PMS2 antibody, DNA mismatch repair gene homologue antibody, H_DJ0042M02.9 antibody, HNPCC4 antibody, PMS 2 antibody, PMS1 protein homolog 2 antibody, PMS2 postmeiotic segregation increased 2 antibody, PMS2CL antibody, PMSL2 antibody

Purification: This is an protein A purified antibody from ascites fluid directed against PMS2-134 and reacts with full length version of PMS2 in human and hamster tissues.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology.

Immunogen Type: Recombinant Protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Application Note: Anti-PMS2 antibody has been tested for use in ELISA and western blotting. This product is suitable for immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user.  Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.38 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PMS2

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Autre désignation: PMS2

Sujet: Background: PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1.  PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.

ID gène: 5395

UniProt: P54278

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response