CENPE anticorps

N° du produit ABIN129515

N° du produit (Fournisseur): 200-301-901

L’anticorps Souris Monoclonal anti-CENPE a été validé pour WB, ELISA, IP, IF et FM. Il convient pour détecter CENPE dans des échantillons de Humain.

Aperçu rapide pour CENPE anticorps (ABIN129515)

Antigène: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CENPE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 1H12

Détail du produit anti-CENPE anticorps

N° du produit (Fournisseur): 200-301-901

Fournisseur: Rockland

Fonction: CENP-E

Réactivité croisée (Details): This Protein A purified antibody is directed against human CENP-E protein.

Attributs du produit: Synonyms: mouse anti-CENP-E antibody, Centromere-associated protein E, Centromeric protein E antibody, KIF10 antibody, Kinesin-7, Kinesin family member 10 antibody, Kinesin-related protein CENPE

Purification: The product was purified from tissue culture supernatant by chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1

Information d'application

Indications d'application:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:500 - 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CENPE

Antigène: CENPE (Centromere Protein E, 312kDa (CENPE))

Autre désignation: CENPE

Sujet: Background: CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G₂ phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase.  CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

ID gène: 1062

UniProt: Q02224

Pathways: M Phase, Maintenance of Protein Location