RAD23A anticorps (AA 115-140)

N° du produit ABIN129556

N° du produit (Fournisseur): 600-101-291

Cet anticorps Chèvre Polyclonal détecte spécifiquement RAD23A dans WB et ELISA. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour RAD23A anticorps (AA 115-140) (ABIN129556)

Antigène: RAD23A (RAD23 Homolog A (RAD23A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD23A est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RAD23A anticorps

Épitope: AA 115-140

N° du produit (Fournisseur): 600-101-291

Fournisseur: Rockland

Fonction: HR23A Antibody

Réactivité croisée (Details): Reactivity occurs against human HR23A protein.

Attributs du produit: Synonyms: goat anti-HR23A Antibody, hHR23A antibody, MGC111083 antibody, RAD23 homolog A antibody, RAD23 yeast homolog A antibody, RAD23A antibody, UV excision repair protein RAD23 homolog A

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to HR23A by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:16,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-RAD23A (ABIN129556)

Zhu, Wani, Arab, El-Mahdy, Ray, Wani: "Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites." dans: DNA repair, Vol. 8, Issue 2, pp. 262-73, (2009) (PubMed).

Détails sur RAD23A

Antigène: RAD23A (RAD23 Homolog A (RAD23A))

Autre désignation: RAD23A

Sujet: Background: HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

ID gène: 5886, 4826964

UniProt: P54725

Pathways: Réparation de l'ADN