Aurora Kinase B anticorps (AA 225-250)

N° du produit ABIN129590

N° du produit (Fournisseur): 600-401-465

L’anticorps Lapin Polyclonal anti-Aurora Kinase B a été validé pour WB, ELISA et FM. Il convient pour détecter Aurora Kinase B dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Aurora Kinase B anticorps (AA 225-250) (ABIN129590)

Antigène: Aurora Kinase B (AURKB)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Aurora Kinase B est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-Aurora Kinase B anticorps

Épitope: AA 225-250

N° du produit (Fournisseur): 600-401-465

Fournisseur: Rockland

Fonction: AURORA KINASE B Antibody

Réactivité croisée (Details): Reactivity occurs against human Aurora Kinase B protein.

Attributs du produit: Synonyms: rabbit anti-Aurora B Antibody, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

Purification: Aurora B antibody is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 225-250 of Human Aurora Kinase B protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Aurora B is affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.   Human brain tissue lysate or HeLa cell lysate can be used as a positive control.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.39 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Anti-Aurora B at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Aurora Kinase B (ABIN129590)

Slawson, Lakshmanan, Knapp, Hart: "A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin." dans: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4130-40, (2008) (PubMed).

Détails sur Aurora Kinase B

Antigène: Aurora Kinase B (AURKB)

Autre désignation: AURKB

Sujet: Background: Aurora Kinase B (also known as Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body.  High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G₂/M phase and expression is up-regulated in cancer cells during M phase.

ID gène: 9212, 83776600

UniProt: Q96GD4

Pathways: TCR Signaling, Cycle Cellulaire, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades