ATF3 anticorps (Internal Region)

N° du produit ABIN129602

N° du produit (Fournisseur): 600-401-493

L’anticorps Lapin Polyclonal anti-ATF3 a été validé pour WB et ELISA. Il convient pour détecter ATF3 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour ATF3 anticorps (Internal Region) (ABIN129602)

Antigène: ATF3 (Activating Transcription Factor 3 (ATF3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATF3 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-ATF3 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-493

Fournisseur: Rockland

Fonction: ATF3 Antibody

Réactivité croisée (Details): This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems.

Attributs du produit: Synonyms: rabbit anti-ATF3 Antibody, ATF 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3

Purification: Affinity purified antibody

Stérilité: Sterile filtered

Immunogène:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATF3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Affinity purified anti-ATF3 has been tested by ELISA and western blotting against recombinant forms of the protein. Although not tested, this antibody is likely function in most immunoassays including immunofluorescence microscopy, immunohistochemistry.

Western Blot Dilution: 1:200 - 1:2000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-ATF3 (ABIN129602)

Xie, Xu, Wu, Shen, Zhao, Du, Lv, Gu, Pan, Xu, Xie, Li: "Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells." dans: The American journal of pathology, Vol. 176, Issue 2, pp. 939-51, (2010) (PubMed).

Détails sur ATF3

Antigène: ATF3 (Activating Transcription Factor 3 (ATF3))

Autre désignation: ATF3

Sujet: Background: ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter.  Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA.  ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter.  Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.

ID gène: 467

NCBI Accession: NP_001025458

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response