HDAC1 anticorps (AA 450-482)

N° du produit ABIN129652

N° du produit (Fournisseur): 600-401-879

Cet anticorps Lapin Polyclonal détecte spécifiquement HDAC1 dans WB, ELISA, IHC, IF, FM et MA. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour HDAC1 anticorps (AA 450-482) (ABIN129652)

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-HDAC1 anticorps

Épitope: AA 450-482

N° du produit (Fournisseur): 600-401-879

Fournisseur: Rockland

Fonction: HDAC1 (C-terminus) Antibody

Réactivité croisée (Details): Anti-HDAC-1 antibody is directed against human HDAC-1 protein.

Attributs du produit: Synonyms: rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody

Purification: HDAC-1 antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-HDAC-1 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user.   Specific nuclear staining is observed by IHC.  Expect bands at 65 kDa in size corresponding to HDAC-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 10 μg / ml

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.33 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-HDAC1 (ABIN129652)

Scharer, Barwick, Guo, Bally, Boss: "Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs." dans: Nature communications, Vol. 9, Issue 1, pp. 1698, (2018) (PubMed).

Nohata, Hanazawa, Kinoshita, Inamine, Kikkawa, Itesako, Yoshino, Enokida, Nakagawa, Okamoto, Seki: "Tumour-suppressive microRNA-874 contributes to cell proliferation through targeting of histone deacetylase 1 in head and neck squamous cell carcinoma." dans: British journal of cancer, Vol. 108, Issue 8, pp. 1648-58, (2013) (PubMed).

Unoki, Nishidate, Nakamura: "ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain." dans: Oncogene, Vol. 23, Issue 46, pp. 7601-10, (2004) (PubMed).

Détails sur HDAC1

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: HDAC1

Sujet: Background: HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

ID gène: 3065, 13128860

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis