PCNA anticorps (Internal Region)

N° du produit ABIN129712

N° du produit (Fournisseur): 600-401-968

Cet anticorps Lapin Polyclonal détecte spécifiquement PCNA dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour PCNA anticorps (Internal Region) (ABIN129712)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-PCNA anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-968

Fournisseur: Rockland

Fonction: PCNA Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human PCNA protein.

Attributs du produit: Synonyms: rabbit anti-PCNA antibody, proliferating cell nuclear antigen, cyclin

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29-36 kDa in size corresponding to PCNA protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:8,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.69 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: Background: The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand.  PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision.  PCNA is a useful marker for DNA synthesis and is highly conserved among most species.

ID gène: 5111, 33239451

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy