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Sca-1/Ly-6A/E anticorps (Biotin)

Cet anticorps anti-Sca-1/Ly-6A/E est un anticorps Rat Monoclonal détectant Sca-1/Ly-6A/E dans FACS. Adapté pour Souris.
N° du produit ABIN135291

Aperçu rapide pour Sca-1/Ly-6A/E anticorps (Biotin) (ABIN135291)

Antigène

Voir toutes Sca-1/Ly-6A/E (Ly6a) Anticorps
Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivité

  • 69
  • 5
  • 2
Souris

Hôte

  • 49
  • 25
Rat

Clonalité

  • 57
  • 16
Monoclonal

Conjugué

  • 18
  • 7
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Sca-1/Ly-6A/E est conjugé à/à la Biotin

Application

  • 47
  • 18
  • 17
  • 17
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

D7
  • Specificité

    Mouse Ly-6A/E (Ly-6A.2 & Ly-6E.1), Mr 18 kDa

    Attributs du produit

    Rat Anti-Mouse Ly-6A/E-BIOT

    Immunogène

    IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L

    Isotype

    IgG2a
  • Indications d'application

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Costim - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY5.5, PACBLU, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Commentaires

    In vitro lymphocyte activation

    Volume d'échantillon

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8°C
  • Antigène

    Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

    Autre désignation

    Ly-6A/E

    Sujet

    Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.

    Pathways

    Sensory Perception of Sound, Activated T Cell Proliferation
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