CD86 anticorps

N° du produit ABIN135432

Cet anticorps anti-CD86 est un anticorps Rat Monoclonal détectant CD86 dans FACS. Adapté pour Souris.

Aperçu rapide pour CD86 anticorps (ABIN135432)

Antigène: CD86

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD86 est non-conjugé

Application: Flow Cytometry (FACS)

Clone: 2D10

Détail du produit anti-CD86 anticorps

Specificité: Mouse CD86, Mr 80 kDa

Attributs du produit: Rat Anti-Mouse CD86-UNLB

Purification: Purified

Immunogène: Mouse B cell lymphoma cell line 5C2

Isotype: IgG2b

Information d'application

Indications d'application:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Sep - Reported in literature , CMCD - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, APC, SPRD, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Commentaires:

In vivo and in vitro blocking assays, Identification of CD86+ cells in frozen section

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Agent conservateur: Without preservative

Conseil sur la manipulation: Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détails sur CD86

Antigène: CD86

Autre désignation: CD86

Sujet: CD86/B7-2 is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80/B7-1, CD86 participates in T cell costimulation via interaction with CD28 and CD152/CTLA-4. It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes and dendritic cells. Since CD86/B7-2 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation