Cyclin E1 anticorps (pThr395)

N° du produit ABIN1531225

L’anticorps anti-Cyclin E1 Polyclonal Lapin est utilisé pour la détection de Cyclin E1 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour Cyclin E1 anticorps (pThr395) (ABIN1531225)

Antigène: Cyclin E1 (CCNE1)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin E1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-Cyclin E1 anticorps

Épitope: AA 361-410, pThr395

Fonction: Cyclin E1 (phospho Thr395) Antibody

Specificité: Phospho-Cyclin E1 (T395) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T395.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr395. AA range:361-410

Isotype: IgG

Information d'application

Indications d'application: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur Cyclin E1

Antigène: Cyclin E1 (CCNE1)

Autre désignation: Cyclin E1

Sujet:

Synonyms: CCNE1, CCNE, G1/S-specific cyclin-E1

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

Gene Name: CCNE1

Protein Name: G1/S-specific cyclin-E1

Poids moléculaire: 47 kDa

ID gène: 898

OMIM: 123837

UniProt: P24864

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases