VEGFA anticorps (Isoform 165)

N° du produit ABIN155173

Cet anticorps Souris Monoclonal détecte spécifiquement VEGFA dans WB, EIA et Func. Il présente une réactivité envers Humain.

Aperçu rapide pour VEGFA anticorps (Isoform 165) (ABIN155173)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VEGFA est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: 3

Détail du produit anti-VEGFA anticorps

Épitope: Isoform 165

Specificité: The antibody recognizes VEGF-A.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography

Immunogène: Recombinant Human VEGF165 protein (45 kDa)

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1-15 μg/mL. Western blot: 2-10 μg/mL. Neutralization: Blocks the VEGF-A induced proliferation of ACE cell at a 1: 6 Molar ratio ofligand to antibody. Inhibits the binding of VEGF-A to the VEGF receptors 1 (Flt-1) and 2 (KDR).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: H2O, Trehalose, Na-Phosphate, Polysorbart20

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody, thought stable at RT, is best stored desiccated below 0 °C. Reconstituted anti-VEGF-A is stable at 2-8 °C for one month or (in aliquots) at - 20 °C for longer.

Détails sur VEGFA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A

Sujet: Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

ID gène: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling