VEGFA anticorps

N° du produit ABIN155190

Cet anticorps anti-VEGFA est un anticorps Lapin Polyclonal détectant VEGFA dans WB, IHC (p) et EIA. Adapté pour Humain et Souris.

Aperçu rapide pour VEGFA anticorps (ABIN155190)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGFA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-VEGFA anticorps

Specificité: The antibody recognizes Human and Murine VEGF-A under reducing and non-reducing conditions.

Réactivité croisée (Details): Species reactivity (tested):Human and Mouse.

Purification: Protein A Chromatography.

Immunogène: Recombinant Murine VEGF164 Dimer (Cat.-No DA3518)

Information d'application

Indications d'application: ELISA: To detect Murine VEGF-A by direct ELISA a concentration of at least 0.5 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 0.5- 1.0 ng/well of recombinant murine VEGF164 or other VEGF-A splice forms. Western blot: To detect murine VEGF-A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections (Pretreatment: 0.01 % Proteinase K, 15 min atRT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives and stabilizers

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur VEGFA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A

Sujet: VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternate transcriptional splice variants, encoding either freely secreted or cell associated isoforms, have been characterized. There is also evidence for the use of non AUG (CUG) translation initiation sites upstream of, and in frame with the first AUG, leading to additional isoforms.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

ID gène: 22339

NCBI Accession: NP_001020421

UniProt: Q00731

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling