SAG anticorps

N° du produit ABIN1580413

Détail du produit anti-SAG anticorps

Antigène: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SAG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: affinity purified antibody

Clone: S128

Isotype: IgG1

Information d'application

Indications d'application: Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal homogenate. The arrestin protein runs at about ~48 kDa on SDS-PAGE gels.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur SAG

Antigène: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Autre désignation: Arrestin-1/ S-antigen (SAG Produits)

Synonymes: anticorps RP47, anticorps S-AG, anticorps MGC75721, anticorps MGC84416, anticorps sag, anticorps zgc:114197, anticorps SAGMR, anticorps SANTI, anticorps ARR, anticorps sag1, anticorps A930001K18Rik, anticorps Arr1, anticorps Irbp, anticorps arrestin, anticorps S-antigen visual arrestin, anticorps S-antigen, anticorps S-antigen; retina and pineal gland (arrestin), anticorps S-antigen; retina and pineal gland (arrestin) S homeolog, anticorps S-antigen; retina and pineal gland (arrestin) b, anticorps S-antigen, retina and pineal gland (arrestin), anticorps S-antigen; retina and pineal gland (arrestin) L homeolog, anticorps SAG, anticorps PF10_0343, anticorps sag, anticorps sag.S, anticorps sagb, anticorps Sag, anticorps sag.L

Sujet: The arrestin proteins are a family of regulators of cell signaling of G protein-coupled receptors (GPCR). Visual arrestin was first discovered as a result of the experimental model of human uveitis, an autoimmune disease of the eye. In this model, called experimental allergic uveitis, animals were injected with extracts made from the retina of the same species mixed with Freund's complete adjuvant. The animals mounted a strong immune response to the extract, and the antibody response was used to identify several immunogenic retinal proteins. One of these was called S-antigen, for soluble antigen. The protein was found to be abundant in retina, about 48 kDa in molecular weight, and localized in the outer segments of the photoreceptors. Several years later, Hermann K�hn and colleagues discovered that this protein binds to phosphorylated rhodopsin and prevents this protein from activating transducin. Transducin is a typical heterotrimeric G protein, composed of alpha, and beta,gamma, subunits. Rhodopsin phosphorylation is mediated by Rhodopsin kinase (a.k.a. GRK1), the prototypic member of a family of GPCR kinases. Since the S-antigen protein arrested the activity of rhodopsin it was renamed arrestin, and became the prototypic member of the arrestin protein family. Subsequently, Robert

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling