Lamin A/C anticorps

N° du produit ABIN1580428

Cet anticorps Poulet Polyclonal détecte spécifiquement Lamin A/C dans WB, IHC, IF et ICC. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Porc et Mammifères.

Aperçu rapide pour Lamin A/C anticorps (ABIN1580428)

Antigène: Lamin A/C (LMNA)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Mammifères

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Lamin A/C est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Lamin A/C anticorps

Purification: IgY preparation

Isotype: IgY

Information d'application

Indications d'application: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:2,000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 20 mg/mL

Buffer: This antibody is an IgY prep at a total protein concentration of 20mg/mL. The preparation contains 10mM sodium azide as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur Lamin A/C

Antigène: Lamin A/C (LMNA)

Autre désignation: Lamin A/C

Sujet: The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm. The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene, while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. This is the case with this polyclonal, which was raised against full length recombinant human Lamin A. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of maturation promoting factor, the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging. The HGNC name for this protein is LMNA.

Pathways: Apoptose, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus