LAMP1 anticorps

N° du produit ABIN1580431

Cet anticorps Souris Monoclonal détecte spécifiquement LAMP1 dans WB, IHC, IF et ICC. Il présente une réactivité envers Humain, Souris, Rat, Porc, Boeuf (Vache) et Mammifères.

Aperçu rapide pour LAMP1 anticorps (ABIN1580431)

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5H6

Détail du produit anti-LAMP1 anticorps

Purification: affinity purified antibody

Isotype: IgG1

Information d'application

Indications d'application: The antibody solution can be used at dilutions of at least 1:2,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: Lamp1

Sujet: As the name suggests, LAMP1 is a protein primarily associated with the lysosomal membrane. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center. The protein is also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA, as the protein was independently discovered and named by several different labs. CD is an abbreviation for Cluster of Differentiation and refers to a protocol for the naming of proteins and other surface markers of human leucocytes defined by binding of specific monoclonal antibodies. LAMP1 is found on the cell surface of lymphocytes undergoing degranulation, a process in which cytoplasmic vesicles fuse with the plasma membrane, and this phenomena resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-terminal region which is inside the lysosome, hence topologically external to the cell, which is often refered to as the lumenal domain. The lumenal domain consists of two homologous globular seqments separated by a proline rich sequence. Next there is a single membrane spanning domain and a short 11 amino acid C-terminal cytoplasmic tail. This tail region contains, at the extreme C-terminus, a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid. This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a native molecular weight of 44.8 kDa. However the N-terminal lumenal segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western blots the protein runs as a diffuse band at 90-120 kDa. The HGNC name for this protein is LAMP1.

Pathways: Autophagy