VEGFR2/CD309 anticorps (Biotin)

N° du produit ABIN1589929

Cet anticorps Rat Monoclonal détecte spécifiquement VEGFR2/CD309 dans WB. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour VEGFR2/CD309 anticorps (Biotin) (ABIN1589929)

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp VEGFR2/CD309 est conjugé à/à la Biotin

Application: Western Blotting (WB)

Clone: 3C8

Détail du produit anti-VEGFR2/CD309 anticorps (Biotin)

Fonction: VEGFR-2/Flk-1 antibody

Specificité: Recombinant mouse soluble Flk-1

Attributs du produit: Chromosomal location: 5 C3.3, 5 42.0 cM
Monoclonal antibodies were produced from a murine hybridoma elicited from a rat immunized with purified, insect cell-derived recombinant mouse soluble Flk-1/KDR (110 kDa) as the immunizing antigen.

Purification: Rat IgG1 antibody from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogène: Recombinant mouse soluble Flk-1

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: use at 2-5 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Date de péremption: 24 months

Détails sur VEGFR2/CD309

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Autre désignation: VEGFR-2/Flk-1

Sujet: Vascular endothelial growth factor receptor-2 , Kdr, Ly73, Flk-1, VEGF receptor 2, kinase insert domain protein receptor,VEGF R2 (mouseFlk1 gene), VEGF R1 (Flt1) and VEGF R3 (Flt4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulinlike repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGF R2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The solube receptor was used as a an antigen.

ID gène: 16542

UniProt: P35918

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling