MAPKAP Kinase 2 anticorps (AA 44-55)
Aperçu rapide pour MAPKAP Kinase 2 anticorps (AA 44-55) (ABIN1590004)
Antigène
Voir toutes MAPKAP Kinase 2 (MAPKAPK2) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- AA 44-55
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Fonction
- MAPKAPK2 (aa44-55)
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Séquence
- PQFHVKSGLQ IK
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Specificité
- This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).
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Réactivité croisée
- Humain, Souris, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotype
- IgG
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Indications d'application
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Western Blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, I
Peptide ELISA: antibody detection limit dilution 1:32000.
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Commentaires
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Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
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Autre désignation
- MAPKAPK2
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Sujet
- MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2
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ID gène
- 9261, 17164, 289014
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NCBI Accession
- NP_004750, NP_116584
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Pathways
- Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
Antigène
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