NAE1 anticorps (AA 401-500) (HRP)

N° du produit ABIN1712107

Cet anticorps Lapin Polyclonal détecte spécifiquement NAE1 dans WB, ELISA, IHC (p) et IHC (fro). Il présente une réactivité envers Souris et Rat.

Aperçu rapide pour NAE1 anticorps (AA 401-500) (HRP) (ABIN1712107)

Antigène: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NAE1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-NAE1 anticorps (HRP)

Épitope: AA 401-500

Réactivité croisée: Souris, Rat

Homologie: Human,Dog,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human APPBP1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur NAE1

Antigène: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Autre désignation: APPBP1

Sujet:

Synonyms: HPP1, ula-1, APPBP1, A-116A10.1, NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1

Background: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

ID gène: 8883

UniProt: Q13564