DPPA3 anticorps (AA 101-159)

N° du produit ABIN1713577

Cet anticorps Lapin Polyclonal détecte spécifiquement DPPA3 dans WB, ELISA, ICC, IHC (p), IF (cc), IF (p) et IHC (fro). Il présente une réactivité envers Souris et Porc et a été mentionné dans 1 publication.

Aperçu rapide pour DPPA3 anticorps (AA 101-159) (ABIN1713577)

Antigène: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Reactivité: Souris, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DPPA3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-DPPA3 anticorps

Épitope: AA 101-159

Réactivité croisée: Souris, Porc

Homologie: Human

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Stella/DPPA3

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-DPPA3 (ABIN1713577)

Wasielak, Więsak, Bogacka, Jalali, Bogacki: "Zygote arrest 1, nucleoplasmin 2, and developmentally associated protein 3 mRNA profiles throughout porcine embryo development in vitro." dans: Theriogenology, Vol. 86, Issue 9, pp. 2254-2262, (2016) (PubMed).

Détails sur DPPA3

Antigène: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Autre désignation: Stella/DPPA3

Sujet:

Synonyms: STELLA, Developmental pluripotency-associated protein 3, Stella-related protein, DPPA3, STELLAR

Background: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

ID gène: 359787

UniProt: Q6W0C5