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DPPA3 anticorps (AA 101-159)

DPPA3 Reactivité: Souris, Porc WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1713577
  • Antigène Voir toutes DPPA3 Anticorps
    DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))
    Épitope
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 101-159
    Reactivité
    • 20
    • 11
    • 1
    Souris, Porc
    Hôte
    • 20
    • 1
    • 1
    Lapin
    Clonalité
    • 22
    Polyclonal
    Conjugué
    • 11
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp DPPA3 est non-conjugé
    Application
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Homologie
    Human
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human Stella/DPPA3
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Wasielak, Więsak, Bogacka, Jalali, Bogacki: "Zygote arrest 1, nucleoplasmin 2, and developmentally associated protein 3 mRNA profiles throughout porcine embryo development in vitro." dans: Theriogenology, Vol. 86, Issue 9, pp. 2254-2262, (2016) (PubMed).

  • Antigène
    DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))
    Autre désignation
    Stella/DPPA3 (DPPA3 Produits)
    Synonymes
    anticorps STELLA, anticorps 2410075G02Rik, anticorps PCG7, anticorps PGC7, anticorps Stella, anticorps developmental pluripotency associated 3, anticorps developmental pluripotency-associated 3, anticorps DPPA3, anticorps Dppa3
    Sujet

    Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

    Subcellular location: Cytoplasm, Nucleus

    Synonyms: STELLA, Developmental pluripotency-associated protein 3, Stella-related protein, DPPA3, STELLAR

    ID gène
    359787
    UniProt
    Q6W0C5
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