EIF2A anticorps (pSer52)

N° du produit ABIN1714407

Cet anticorps anti-EIF2A est un anticorps Lapin Polyclonal détectant EIF2A dans WB, ELISA, FACS, IHC (p), IF (cc), IF (p) et IHC (fro). Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour EIF2A anticorps (pSer52) (ABIN1714407)

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2A est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-EIF2A anticorps

Épitope: pSer52

Réactivité croisée: Humain, Souris, Rat

Homologie: Cow

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR]

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-EIF2A (ABIN1714407)

Cha, Song, Kang, Park, Park, Kim, Shim, Kim: "miR-211 Plays a Critical Role in Cnidium officinale Makino Extract-Induced, ROS/ER Stress-Mediated Apoptosis in U937 and U266 Cells." dans: International journal of molecular sciences, Vol. 19, Issue 3, (2018) (PubMed).

Yao, Lu, Hu, Yu, Chen, Wang: "A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure." dans: Nature communications, Vol. 8, Issue 1, pp. 133, (2017) (PubMed).

He, Long, Zhong, Fu, Li, Lin: "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." dans: Molecular and cellular biochemistry, Vol. 389, Issue 1-2, pp. 151-8, (2014) (PubMed).

Détails sur EIF2A

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Autre désignation: eIF2 alpha

Sujet:

Synonyms: EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF2S1

Background: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

ID gène: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly