DNMT1 anticorps (AA 637-650)

N° du produit ABIN1721507

L’anticorps anti-DNMT1 Monoclonal Souris est utilisé pour la détection de DNMT1 dans des échantillons de Humain, Souris et Poisson zèbre (Danio rerio). Il a été validé pour WB et ELISA.

Aperçu rapide pour DNMT1 anticorps (AA 637-650) (ABIN1721507)

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivité: Humain, Souris, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNMT1 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 4G11-C7

Détail du produit anti-DNMT1 anticorps

Épitope: AA 637-650

Specificité: Reacts with human, mouse and zebrafish DNMT1.

Purification: Purified (protein G)

Immunogène: Synthetic peptide corresponding to aa 637-650 of human DNMT1 (accession no. NP_001370).

Isotype: IgG2b

Information d'application

Indications d'application: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(2 μg/mL)

Positive control: ES and H1299 cell lysates.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS (2 mg/mL), pH 7.4.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute in PBS or medium which is identical to that used in the assay system.

Stock: -20 °C

Stockage commentaire: This antibody is stable for at least one year at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur DNMT1

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNMT1

Sujet: Methylation of DNA at cytosine residues is important in regulation of gene expression and genomic imprinting. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked to development of cancer. Thus far three families of mammalian DNA methyltransferase genes have been identified : DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.

DNA (cytosine-5)-methyltransferase 1 CXXC-type zinc finger protein 9, CXXC9 DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI Gene name : DNMT1

ID gène: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2