PCNA anticorps (AA 53-196)

N° du produit ABIN1724759

L’anticorps Souris Monoclonal anti-PCNA a été validé pour WB, IHC, ELISA et FACS. Il convient pour détecter PCNA dans des échantillons de Humain et Singe. Il y a 2+ publications disponibles.

Aperçu rapide pour PCNA anticorps (AA 53-196) (ABIN1724759)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 7H4F8

Détail du produit anti-PCNA anticorps

Épitope: AA 53-196

Fonction: PCNA Antibody

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-PCNA (ABIN1724759)

Takawa, Cho, Hayami, Toyokawa, Kogure, Yamane, Iwai, Maejima, Ueda, Masuda, Dohmae, Field, Tsunoda, Kobayashi, Akasu, Sugiyama, Ohnuma, Atomi, Ponder, Nakamura, Hamamoto: "Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression." dans: Cancer research, Vol. 72, Issue 13, pp. 3217-27, (2012) (PubMed).

Zhao, Ho, Lo, Espejo, Bedford, Hung, Wang: "Interaction of proliferation cell nuclear antigen (PCNA) with c-Abl in cell proliferation and response to DNA damages in breast cancer." dans: PLoS ONE, Vol. 7, Issue 1, pp. e29416, (2012) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Poids moléculaire: 28.7 kDa

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy