NCK1 anticorps

N° du produit ABIN1774727

Il existe 1 publication pour ce produit. L’anticorps anti-NCK1 Monoclonal Souris est utilisé pour la détection de NCK1 dans des échantillons de Humain et Souris. Il a été validé pour WB et ICC.

Aperçu rapide pour NCK1 anticorps (ABIN1774727)

Antigène: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NCK1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: EM-06

Détail du produit anti-NCK1 anticorps

Fonction: Anti-NCK1 Purified

Réactivité croisée (Details): Human, Mouse

Attributs du produit: The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Purified recombinant second SH3 domain of human Nck1 fused to GST

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 2 μg/mL, positive control: Jurkat cell lysate.
Immunocytochemistry: Recommended dilution: 2 μg/mL, positive control: human NCK1-transfected COS-7 cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-NCK1 (ABIN1774727)

Borroto, Arellano, Dopfer, Prouza, Suchànek, Fuentes, Orfao, Schamel, Alarcón: "Nck recruitment to the TCR required for ZAP70 activation during thymic development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1103-12, (2013) (PubMed).

Détails sur NCK1

Antigène: NCK1 (NCK Adaptor Protein 1 (NCK1))

Autre désignation: NCK1

Sujet: NCK adaptor protein 1,NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.,NCK-1, NCKalpha

ID gène: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals