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Nestin anticorps (FITC)

NES Reactivité: Humain IF, ICC Hôte: Souris Monoclonal 10C2 FITC
N° du produit ABIN1774766
  • Antigène Voir toutes Nestin (NES) Anticorps
    Nestin (NES)
    Reactivité
    • 131
    • 69
    • 51
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 103
    • 38
    • 4
    • 2
    • 1
    Souris
    Clonalité
    • 108
    • 40
    Monoclonal
    Conjugué
    • 66
    • 10
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Cet anticorp Nestin est conjugé à/à la FITC
    Application
    • 82
    • 51
    • 49
    • 48
    • 40
    • 40
    • 39
    • 28
    • 25
    • 15
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    This antibody recognises nestin, a member of the intermediate filament family required for brain and eye development.
    Purification
    Protein G purified
    Immunogène
    A 150-amino-acid fragment from the cloned human nestin conjugated to GST
    Clone
    10C2
    Isotype
    IgG1
    Top Product
    Discover our top product NES Anticorps primaire
  • Indications d'application
    Optimal antibody dilution should be determined by titration.
    Restrictions
    For Research Use only
  • Validation #029824 (Immunofluorescence)
    'Independent Validation' signe
    by
    Ensigna Biosystems
    No.
    #029824
    Date
    06.11.2014
    Antigène
    Numéro du lot
    I030314
    Application validée
    Immunofluorescence
    Contrôle positif
    PC-3 cells
    Contrôle négative
    MCF-7 cells
    Conclusion
    Faint fluorescent signal was detected in the positive control cells. No signal was detected in the negative control cells or isotype negative control.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: Nestin (NES) (FITC)
    • Catalog number: ABIN1774766
    • Lot number: I030314
    • Dilution: 1:75, 1:100
    Anticorps secondaire
    Full Protocol
    • Prostate and breast adenocarcinoma cell lines were grown directly on chamber slides, washed with 1X PBS, and fixed with 4% paraformaldehyde in 1X PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1X PBS / 5% normal goat serum / 0.3% Triton X-100 for 60 min at RT.
    • Cells were rinsed two times in 1X PBS.
    • Cells were incubated with primary antibody diluted 1:75 and 1:100 in 1X PBS / 1% BSA / 0.3% Triton X-100 overnight at 4°C in dark conditions.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with DAPI-Fluoromount-G.
    • Stained cells were imaged with a Nikon Eclipse E600 microscope and Olympus DP70 camera.
    Notes
    The anti-Nestin antibody showed positive staining of weak intensity localized appropriately to the cell cytoplasm in a pattern consistent with intermediate filaments in positive control PC3 cells. No staining was seen in the negative control MCF7 cells or with the isotype control antibody. Although specificity of the antibody was acceptable, its sensitivity with the current protocol was questionable. It is, however, entirely possible that if this antibody is used with an appropriate amplification protocol the sensitivity would be increased.
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Purified IgG conjugated to Fluoroscein using Innova Biosciences Lightning-Link®, supplied in Phosphate buffered saline (PBS) containing 0.09 % Sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antigène
    Nestin (NES)
    Autre désignation
    Nestin (NES Produits)
    Synonymes
    anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes
    Sujet
    Neural Stem Cell Marker
    ID gène
    407242
    OMIM
    600915
    UniProt
    P18175
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