Calreticulin anticorps (Internal Region)

N° du produit ABIN1782157

Cet anticorps Chèvre Polyclonal détecte spécifiquement Calreticulin dans WB et ELISA. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour Calreticulin anticorps (Internal Region) (ABIN1782157)

Antigène: Calreticulin (CALR)

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Calreticulin est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-Calreticulin anticorps

Épitope: Internal Region

Fonction: calreticulin

Séquence: HPEIDNPEYS PDPS

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in Human and Rat Lung lysates (calculated MW of 48.1 kDa according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14,270(28)

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Calreticulin

Antigène: Calreticulin (CALR)

Autre désignation: CALR

Sujet: CALR, calreticulin, CRT, RO, SSA, cC1qR, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro, calreticulin), calregulin, endoplasmic reticulum resident protein 60, grp60

Poids moléculaire: Expected molecular weight 60 kDa

ID gène: 811, 12317, 64202

NCBI Accession: NP_004334

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response