MICA anticorps

N° du produit ABIN180911

L’anticorps anti-MICA Monoclonal Souris est utilisé pour la détection de MICA dans des échantillons de Humain. Il a été validé pour FACS, IP, IHC (fro) et Func.

Aperçu rapide pour MICA anticorps (ABIN180911)

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICA est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 6D4

Détail du produit anti-MICA anticorps

Specificité: This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).

Réactivité croisée (Details): Also reacts to: MICB

Attributs du produit: Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

Purification: Affinity chromatography on Protein G

niveau d'endotoxine: Low Endotoxin

Immunogène: MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused withcells of the P3 mouse myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Immunoprecipitation. Functional Assays. Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur MICA

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: MICA

Sujet: MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

ID gène: 4276

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response