Leptin anticorps

N° du produit ABIN181148

L’anticorps anti-Leptin Monoclonal Souris est utilisé pour la détection de Leptin dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour Leptin anticorps (ABIN181148)

Antigène: Leptin (LEP)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Leptin est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Leptin anticorps

Specificité: This antibody detects Leptin.

Attributs du produit: Synonyms: LEP, OB, OBS, Obesity factor, Obese protein

Purification: Affinity Chromatography on Protein A

Immunogène: Highly pure (>98%) recombinant Human Leptin

Isotype: IgG1

Information d'application

Indications d'application: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect recombinant human Leptin when used with Biotin conjugatedanti-Human Leptin antibody (cat. PP1041B) as the detection antibody at a concentration ofapproximately 0.5-1.0 μg/mL. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at aconcentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Leptin

Antigène: Leptin (LEP)

Autre désignation: Leptin

Sujet: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

ID gène: 3952

UniProt: P41159

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process