CLCF1
Reactivité: Humain
Hôte: Lapin
Polyclonal
FITC
Indications d'application
ELISA: Indirect: To detect hNNT-1/BCSF-3 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNNT-1/BCSF-3. Sandwich: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human NNT-1/BCSF-3 as a detection antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. NNT1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. NNT1 may act as a neuroimmunoendocrine modulator of hypothalamus-pituitary-adrenal axis stress response.Synonyms: B-cell-stimulating factor 3, BSF3, CLC, Cardiotrophin-like cytokine factor 1, NNT1, Novel neurotrophin-1