IGF1R anticorps (Chain alpha)

N° du produit ABIN181732

Cet anticorps Souris Monoclonal détecte spécifiquement IGF1R dans WB, FACS et IHC (p). Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour IGF1R anticorps (Chain alpha) (ABIN181732)

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IGF1R est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1H7

Détail du produit anti-IGF1R anticorps

Épitope: Chain alpha

Specificité: This antibody recognises CD221. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221. We recommend the use of BM2226LE for this purpose.

Purification: Affinity Chromatography on Protein G

Immunogène: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot. Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Paraffin Sections (1/10-1/40): Requires protein digestionpre-treatment. Recommended Positive Control: Pancreas, placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-IGF1R (ABIN181732)

Beuschlein, Jakoby, Mentz, Zambetti, Jung, Reincke, Süss, Hantel: "IGF1-R inhibition and liposomal doxorubicin: Progress in preclinical evaluation for the treatment of adrenocortical carcinoma." dans: Molecular and cellular endocrinology, Vol. 428, pp. 82-8, (2016) (PubMed).

Hantel, Lewrick, Reincke, Süss, Beuschlein: "Liposomal doxorubicin-based treatment in a preclinical model of adrenocortical carcinoma." dans: The Journal of endocrinology, Vol. 213, Issue 2, pp. 155-61, (2012) (PubMed).

Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." dans: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, (2010) (PubMed).

Détails sur IGF1R

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Autre désignation: CD221 / IGF1R

Sujet: CD221 is a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor

ID gène: 3480

NCBI Accession: NP_000866

UniProt: P08069

Pathways: Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy