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IGF1R anticorps (AA 251-350)

IGF1R Reactivité: Humain, Souris, Rat, Chien FACS Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN726575
  • Antigène Voir toutes IGF1R Anticorps
    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
    Épitope
    • 26
    • 24
    • 24
    • 13
    • 13
    • 11
    • 10
    • 10
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 251-350
    Reactivité
    • 197
    • 117
    • 98
    • 15
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Chien
    Hôte
    • 180
    • 31
    • 2
    • 1
    Lapin
    Clonalité
    • 182
    • 32
    Polyclonal
    Conjugué
    • 121
    • 10
    • 9
    • 9
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Cet anticorp IGF1R est non-conjugé
    Application
    • 132
    • 73
    • 64
    • 61
    • 32
    • 31
    • 17
    • 13
    • 13
    • 10
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
     Réactivité croisée
    Chien, Humain, Souris, Rat
    Homologie
    Cow,Sheep,Pig,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human IGF-1R alpha chain
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #029750 (Western Blotting)
    'Independent Validation' signe
    by
    Alamo Laboratories Inc
    No.
    #029750
    Date
    01.07.2014
    Antigène
    Numéro du lot
    130322
    Application validée
    Western Blotting
    Contrôle positif
    MCF-7 cells – high expression
    Contrôle négative
    PC3 cells – low expression
    Conclusion
    Two strong bands were observed in the positive control, one at the expected molecular weight, and one slightly higher (which may represent a glycosylated or precursor form). Weaker bands of the same molecular weight appear in the negative control, which is consistent with the expected lower expression in PC3 cells. No other major bands are present in either positive or negative controls.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R) (1:200 dilution)
    • Catalog number: ABIN726575
    • Lot number: 130322
    Anticorps secondaire
    • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
    • Lot number: L170-6515
    Full Protocol
    • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
    • 46 μg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
    • The Spectra Multicolor Broad Range (Thermo Scientific, Cat # 26634) were used as molecular mass markers.
    • Proteins were transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
    • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
    • The membrane was rinsed with TBST once.
    • The membrane was immersed with the protein side up in the primary antibody solution (anti-IGF1R; 1:200) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 hours at 4°C.
    • The membrane was rinsed in TBST three times for 5 min each.
    • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • The membrane was rinsed three times with TBST for 5 min each.
    • The membrane was rinsed in TBS twice for 30 s each.
    • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
    • The membrane was rinsed three times with TBST, then incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
    • The membrane was washed in TBST 2 times for 10 min each.
    • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
    Notes
    - No challenges noted.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Hu, Li, Hu, Wu, Li, Meng: "MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene." dans: Growth factors (Chur, Switzerland), Vol. 32, Issue 1, pp. 27-33, (2014) (PubMed).

    Yang, Liu, Li, Wen, Zhu, Xu: "Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis." dans: Cell biochemistry and biophysics, (2014) (PubMed).

  • Antigène
    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
    Autre désignation
    IGF1R (IGF1R Produits)
    Synonymes
    anticorps CMT2N, anticorps CD221, anticorps IGFIR, anticorps IGFR, anticorps JTK13, anticorps IGFIRC, anticorps IGFR1, anticorps IGF-1R, anticorps IGF1R, anticorps DKFZp469I0618, anticorps igf-1r, anticorps xIGF-1R, anticorps xIGFR, anticorps xigf1r, anticorps A330103N21Rik, anticorps D930020L01, anticorps hyft, anticorps igf-ir, anticorps IGF-IR, anticorps IGF-IRb, anticorps wu:fb03h06, anticorps IGF-IRa, anticorps sb:eu1090, anticorps wu:fi20b10, anticorps alanyl-tRNA synthetase, anticorps insulin like growth factor 1 receptor, anticorps insulin-like growth factor 1 receptor, anticorps insulin-like growth factor 1, anticorps insulin-like growth factor I receptor, anticorps insulin like growth factor 1 receptor S homeolog, anticorps insulin-like growth factor 1b receptor, anticorps insulin-like growth factor 1a receptor, anticorps AARS, anticorps IGF1R, anticorps Igf1r, anticorps igf1r, anticorps Igf1, anticorps LOC780503, anticorps LOC100136427, anticorps igf1r.S, anticorps igf1rb, anticorps igf1ra
    Sujet

    Synonyms: IGFR, CD221, IGFIR, JTK13, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R

    Background: When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

    ID gène
    3480
    UniProt
    P08069
    Pathways
    Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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