JUP anticorps (C-Term)

N° du produit ABIN184775

L’anticorps Chèvre Polyclonal anti-JUP a été validé pour WB, IHC et ELISA. Il convient pour détecter JUP dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour JUP anticorps (C-Term) (ABIN184775)

Antigène: JUP (Junction Plakoglobin (JUP))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp JUP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-JUP anticorps

Épitope: C-Term

Fonction: Plakoglobin / Gamma-catenin

Séquence: GLRPPYPTAD HMLA

Specificité: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Réactivité croisée: Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur JUP

Antigène: JUP (Junction Plakoglobin (JUP))

Autre désignation: JUP

Sujet: JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa

ID gène: 3728, 16480

NCBI Accession: NP_002221, NP_068831

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location