VPS35 anticorps (C-Term)

N° du produit ABIN185182

Cet anticorps Chèvre Polyclonal détecte spécifiquement VPS35 dans WB, IHC, IF et ELISA. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 2+ publications.

Aperçu rapide pour VPS35 anticorps (C-Term) (ABIN185182)

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp VPS35 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Classe de qualité: Verified

Détail du produit anti-VPS35 anticorps

Épitope: C-Term

Fonction: VPS35 / MEM3

Séquence: SPESEGPIYE GLIL

Specificité: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

Western Blot: Approx 90 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85 kDa observed in lysates of cell line HEK293 (calculated MW of 91.7 kDa according to Human NP_060676.2, Mouse NP_0

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-VPS35 (ABIN185182)

Klinger, Højland, Jain, Kjolby, Madsen, Svendsen, Olivecrona, Bonifacino, Nielsen: "Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells." dans: The FEBS journal, Vol. 283, Issue 13, pp. 2476-93, (2017) (PubMed).

Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, (2012) (PubMed).

Détails sur VPS35

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Autre désignation: VPS35

Sujet: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35

ID gène: 55737, 65114

NCBI Accession: NP_060676