VPS35 anticorps (C-Term)

N° du produit ABIN185182

Détail du produit anti-VPS35 anticorps

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp VPS35 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Fonction: VPS35 / MEM3

Séquence: SPESEGPIYE GLIL

Specificité: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

Western Blot: Approx 90 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85 kDa observed in lysates of cell line HEK293 (calculated MW of 91.7 kDa according to Human NP_060676.2, Mouse NP_0

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-VPS35 (ABIN185182)

Klinger, Højland, Jain, Kjolby, Madsen, Svendsen, Olivecrona, Bonifacino, Nielsen: "Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells." dans: The FEBS journal, Vol. 283, Issue 13, pp. 2476-93, (2017) (PubMed).

Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, (2012) (PubMed).

Détails sur VPS35

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Autre désignation: VPS35 (VPS35 Produits)

Synonymes: anticorps MEM3, anticorps PARK17, anticorps vps35, anticorps MGC81959, anticorps bZ34G2.3, anticorps si:rp71-34g2.3, anticorps wu:fa66e03, anticorps wu:fb95c08, anticorps zgc:136268, anticorps 23.m05999, anticorps Mem3, anticorps VPS35, anticorps DKFZp459E1510, anticorps CG5625, anticorps DVps35, anticorps Dmel\\CG5625, anticorps Dvps35, anticorps dvps35, anticorps AI647796, anticorps mem3, anticorps VPS35, retromer complex component, anticorps VPS35 retromer complex component L homeolog, anticorps VPS35 retromer complex component, anticorps vacuolar protein sorting 35 homolog (S. cerevisiae), anticorps vacuolar protein sorting-associated protein 35, anticorps vacuolar protein sorting 35, anticorps Vacuolar protein sorting 35, anticorps hypothetical protein, anticorps VPS35 retromer complex component S homeolog, anticorps retromer complex subunit Vps35, anticorps VPS35, anticorps vps35.L, anticorps vps35, anticorps Tc00.1047053511367.170, anticorps Tb10.70.5460, anticorps BBOV_IV009740, anticorps CC1G_12185, anticorps PTRG_08682, anticorps CMU_017400, anticorps PHATR_43830, anticorps MCYG_00751, anticorps MGYG_01212, anticorps EHI_086580, anticorps EHI_002990, anticorps EHI_041950, anticorps EHI_096520, anticorps Bm1_44045, anticorps LOC100285267, anticorps Vps35, anticorps PGTG_10018, anticorps Tsp_09825, anticorps vps35.S

Sujet: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35

ID gène: 55737, 65114

NCBI Accession: NP_060676