RANBP1 anticorps (C-Term)

N° du produit ABIN185257

Cet anticorps Chèvre Polyclonal détecte spécifiquement RANBP1 dans WB, ELISA, IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour RANBP1 anticorps (C-Term) (ABIN185257)

Antigène: RANBP1 (RAN Binding Protein 1 (RANBP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RANBP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-RANBP1 anticorps

Épitope: C-Term

Fonction: RANBP1

Séquence: LSVKEETKED AEEKQ

Specificité: This antibody is expected to recognise reported isoforms 1, 2, 3 and 4.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-LSVKEETKEDAEEKQ

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx. 26-27 kDa band observed in lysates of cell lines A431 HeLa and MCF7 and in Human Duodenum lysates, (calculated MW of 23.3 kDa according to NP_002873.1). This molecular weight is routinely observed by other sources. Recommended concent

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentrati

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur RANBP1

Antigène: RANBP1 (RAN Binding Protein 1 (RANBP1))

Autre désignation: RANBP1

Sujet: RANBP1, RAN binding protein 1, HTF9A, MGC88701

ID gène: 5902

NCBI Accession: NP_002873