PD-L1 anticorps (C-Term)

N° du produit ABIN185339

L’anticorps Chèvre Polyclonal anti-PD-L1 a été validé pour WB, FACS, ELISA et IF. Il convient pour détecter PD-L1 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour PD-L1 anticorps (C-Term) (ABIN185339)

Antigène: PD-L1 (CD274 (PD-L1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PD-L1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-PD-L1 anticorps

Épitope: C-Term

Fonction: CD274 / PD-L1

Séquence: CKKQSDTHLE ET

Specificité: This antibody is expected to recognize reported isoforms a and b (NP_054862.1, NP_001254635.1) only.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 50 kDa band observed in Human Heart lysates and in lysates of cell line U2OS (calculated MW of 33.3 kDa according to 1NP_054862.1).This band was successfully blocked by incubation with the immunizing peptide and is routinely observed

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in th membrane and cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PD-L1 (ABIN185339)

Chen, Li, Meng, Fan, Song, Wang, Zhu, Guo, Zhang, Shi: "Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas." dans: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 1, pp. 101-8, (2012) (PubMed).

Détails sur PD-L1

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: CD274

Sujet: CD274, CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274 Molecule, MGC142294, MGC142296

ID gène: 29126

NCBI Accession: NP_054862, NP_001254635

Pathways: Cancer Immune Checkpoints