HNF4A anticorps (N-Term)

N° du produit ABIN185378

L’anticorps Chèvre Polyclonal anti-HNF4A a été validé pour WB, ELISA et IHC. Il convient pour détecter HNF4A dans des échantillons de Humain.

Aperçu rapide pour HNF4A anticorps (N-Term) (ABIN185378)

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HNF4A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-HNF4A anticorps

Épitope: N-Term

Fonction: HNF4A

Séquence: RLSKTLVDMD MADY

Specificité: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).

Réactivité croisée: Chien, Humain, Porc

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1, NP_000448.3, NP_849181.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2 μg/mL.

Western Blot: Approx 55 kDa and 48 kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8 kDa according to Human NP_000448.3 , 46.6 kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur HNF4A

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Autre désignation: HNF4A

Sujet: HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha

ID gène: 3172

NCBI Accession: NP_849180, NP_000448, NP_849181

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process